Journal: Scientific Reports

Article Title: Cold atmospheric microwave plasma (CAMP) stimulates dermal papilla cell proliferation by inducing β-catenin signaling

doi: 10.1038/s41598-023-30122-z

Figure Lengend Snippet: The CAMP promotes hDPC proliferation. ( A ) The cell viability of hDPCs against PAM. The hDPCs were grown for 15 min in the presence of PAM treated with CAMP at 30W (Low), 35W (Medium), and 40W (High) for 10, 20, and 40 s, respectively. The cells were replaced with fresh media and further grown for 18 h. MTT assay was performed as described in the method section. Ctrl represents non-treated hDPCs, whereas GAM indicates gas-activating media for 40 s. All data are shown as the mean ± SD of experiments conducted in triplicate. Statistical significance; ** P < 0.01. ( B ) Schematic of CAMP treatment in hDPCs and HaCaT cells.

Article Snippet: Human hair DPCs (hDPCs) were purchased from ScienCell (Cat. #2400, CA, USA).

Techniques: MTT Assay

Journal: Scientific Reports

Article Title: Cold atmospheric microwave plasma (CAMP) stimulates dermal papilla cell proliferation by inducing β-catenin signaling

doi: 10.1038/s41598-023-30122-z

Figure Lengend Snippet: PAM upregulates protein expression of the β-catenin signaling pathway and YAP/TAZ and enhances β-catenin translocation in hDPCs. ( A ) Representative immunoblots and ( B ) Relative fold-change expressions of β-catenin, cyclin D1, c-Myc, YAP, and TAZ in PAM-treated hDPCs, after 18 h of incubation. ( C ) Representative immunoblots and relative fold-change expression of p-β-catenin (S552)/β-catenin level in PAM-treated hDPCs, compared to gas control, at 15 min, 1 h, and 2 h of incubation times. ( D ) Representative images of β-catenin immunofluorescence staining and quantitative nuclear β-catenin fluorescence intensity of GAM and PAM against hDPCs for 15 min incubation. The cells were observed under a confocal microscope with 20 × magnification. The scale bar indicates a 50 µm distance. ( E ) The mRNA expressions of LEF1 genes in PAM-treated hDPCs, after 18 h of incubation. All data are shown as the mean ± SD of experiments conducted in triplicate. Statistical significance; ** P < 0.01; *** P < 0.001, and **** P < 0.0001. ns indicates not significant ( P > 0.05).

Article Snippet: Human hair DPCs (hDPCs) were purchased from ScienCell (Cat. #2400, CA, USA).

Techniques: Expressing, Translocation Assay, Western Blot, Incubation, Control, Immunofluorescence, Staining, Fluorescence, Microscopy

Journal: Scientific Reports

Article Title: Cold atmospheric microwave plasma (CAMP) stimulates dermal papilla cell proliferation by inducing β-catenin signaling

doi: 10.1038/s41598-023-30122-z

Figure Lengend Snippet: PAM impeded β-catenin degradation via USP47-regulated β-catenin ubiquitination in hDPCs. ( A ) The mRNA expressions of FER and USP47 genes in PAM-treated hDPCs, after 18 h of incubation. ( B ) Representative immunoblots and (C) Relative fold-change expressions of USP47 and p-β-catenin (Ser33/37/Thr41)/ β-catenin in PAM-treated hDPCs at 15 min, 1 h, and 2 h of incubation times. ( D ) The co-immunoprecipitation between p-β-catenin (Ser33/37/Thr41) and ubiquitin [Ub (HA)] in PAM-treated hDPCs at 15 min of incubation. ( E ) Representative images of USP47 and β-catenin immunofluorescence staining and quantitative analysis of USP47 and β-catenin co-localization in PAM-treated hDPCs compared to GAM control, after 15 min of incubation. The cells were observed under a confocal microscope with 20 × magnification. The scale bar indicates a 20 µm distance. All data are shown as the mean ± SD of experiments conducted in triplicate. Statistical significance; * P < 0.05; *** P < 0.001, and **** P < 0.0001. ns indicates not significant ( P > 0.05).

Article Snippet: Human hair DPCs (hDPCs) were purchased from ScienCell (Cat. #2400, CA, USA).

Techniques: Ubiquitin Proteomics, Incubation, Western Blot, Immunoprecipitation, Immunofluorescence, Staining, Control, Microscopy

Journal: Scientific Reports

Article Title: Cold atmospheric microwave plasma (CAMP) stimulates dermal papilla cell proliferation by inducing β-catenin signaling

doi: 10.1038/s41598-023-30122-z

Figure Lengend Snippet: PAM increases INPP5D mRNA expression and induces Akt and GSK-3β phosphorylation in hDPCs. (A) The heatmap results of human PI3K signaling TaqMan™ mRNA array of PAM-treated hDPCs. ( B ) The mRNA expression of INPP5D gene in PAM-treated hDPCs. ( C ) Representative immunoblots and relative fold-change expressions of p-Akt (Ser473)/ Akt in GAM and PAM- treated hDPCs. Cells were pre-treated with Akt1/2 kinase inhibitor (5 µM) for 1 h, and then treated with GAM or PAM for 15 min. The cell lysates were subjected to Western blot analysis. ( D ) Representative immunoblots and relative fold-change expressions of p-GSK-3α/β (Ser21/9) in GAM and PAM-treated hDPCs. All data are shown as the mean ± SD of experiments conducted in triplicate. Statistical significance; * P < 0.05; ** P < 0.01 and *** P < 0.001.

Article Snippet: Human hair DPCs (hDPCs) were purchased from ScienCell (Cat. #2400, CA, USA).

Techniques: Expressing, Phospho-proteomics, Western Blot

Journal: Scientific Reports

Article Title: Cold atmospheric microwave plasma (CAMP) stimulates dermal papilla cell proliferation by inducing β-catenin signaling

doi: 10.1038/s41598-023-30122-z

Figure Lengend Snippet: Conditioned media (CM) of hDPCs promotes protein expression of the β-catenin signaling pathway and YAP/TAZ in PAM-treated HaCaT cells. ( A ) The characteristics of hDPCs and HaCaT cells after 8 days of co-culturing and treating with PAM. The cell was visualized by Nikon Eclipse Ti-U inverted microscope with 4 × magnification. ( B ) The fluorescence images of GFP hDPCs and Hoechst 33,342 of the co-cultured PAM-treated hDPCs and HaCaT cells. The cells were observed under a confocal microscope with 10 × magnification. The scale bar indicates a 100 µm distance. ( C ) The mRNA expressions of versican genes in PAM-treated hDPCs, after 18 h of incubation. ( D ) Representative immunoblots and (E) Relative fold-change expressions of β-catenin, cyclin D1, c-Myc, YAP, and TAZ in HaCaT cells, after being treated with CM-derived hDPCs for 18 h. All data are shown as the mean ± SD of experiments conducted in triplicate. Statistical significance; * P < 0.05; ** P < 0.01, and *** P < 0.001.

Article Snippet: Human hair DPCs (hDPCs) were purchased from ScienCell (Cat. #2400, CA, USA).

Techniques: Expressing, Inverted Microscopy, Fluorescence, Cell Culture, Microscopy, Incubation, Western Blot, Derivative Assay

Journal: Scientific Reports

Article Title: Cold atmospheric microwave plasma (CAMP) stimulates dermal papilla cell proliferation by inducing β-catenin signaling

doi: 10.1038/s41598-023-30122-z

Figure Lengend Snippet: A schematic representation of the proposed molecular mechanisms of CAMP promoting hDPCs induction.

Article Snippet: Human hair DPCs (hDPCs) were purchased from ScienCell (Cat. #2400, CA, USA).

Techniques: